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What is the primary reason that a modern transmission electron microscope (TEM) can resolve biological images as small as 2 nm, whereas 200 nm is the limit of resolution for a light microscope?


A) Diffraction of light by a light microscope distorts the image.
B) Contrast is enhanced by staining with atoms of heavy metal.
C) Electron beams have much shorter wavelengths than visible light.
D) TEM is used to visualize very thin sections of cells.

E) A) and B)
F) A) and C)

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Tay-Sachs disease is a human genetic abnormality that results in cells accumulating very large, complex, undigested lipids. Which cellular organelle must be defective in this condition?


A) the endoplasmic reticulum
B) the Golgi apparatus
C) the lysosome
D) mitochondrion

E) A) and B)
F) A) and C)

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A mutation that disrupts the ability of an animal cell to add polysaccharide modifications to proteins would most likely cause defects in its


A) nuclear lamina
B) nuclear lamina and extracellular matrix.
C) smooth ER and Golgi apparatus.
D) Golgi apparatus and extracellular matrix.

E) A) and D)
F) A) and C)

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The membranes of various organelles in the endomembrane display differences in their lipid and protein compositions. These differences are largely established by


A) the transport and exchange of membrane lipids among organelles of the endomembrane system by small membrane vesicles.
B) the function of the Golgi apparatus in modifying, sorting, and directing membrane components to their final destinations.
C) the modification of membrane components once they reach their final destination.
D) the synthesis of different lipids and proteins in each of the organelles of the endomembrane system.

E) C) and D)
F) All of the above

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Prokaryotes are classified as belonging to two different domains. What are the domains?


A) Bacteria and Eukarya
B) Bacteria and Protista
C) Archaea and Protista
D) Bacteria and Archaea

E) A) and C)
F) C) and D)

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If plant cells are grown on media containing radioactively labeled thymine for one generation, where will radioactively labeled macromolecules be detected?


A) only in the nucleus
B) only in the nucleus and mitochondria
C) only in the nucleus and chloroplasts
D) in the nucleus, mitochondria, and chloroplasts

E) A) and B)
F) None of the above

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Large numbers of ribosomes are present in cells that specialize in producing which of the following molecules?


A) lipids
B) glycogen
C) proteins
D) cellulose
E) nucleic acids

F) A) and B)
G) A) and C)

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What types of proteins are synthesized by the rough ER?


A) ribosomal proteins
B) soluble cytosolic proteins
C) mitochondrial proteins
D) lysosomal proteins

E) A) and B)
F) C) and D)

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The liver is involved in detoxification of many poisons and drugs. Which of the following structures is primarily involved in this process and therefore abundant in liver cells?


A) rough ER
B) smooth ER
C) Golgi apparatus
D) nuclear envelope
E) lysosomes

F) B) and E)
G) C) and D)

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The cell walls of bacteria, fungi, and plant cells and the extracellular matrix of animal cells are all external to the plasma membrane. Which of the following is a characteristic common to all of these extracellular structures?


A) They have pores that regulate the exchange of water and small molecules between cells and their environment.
B) They are composed of complex mixtures of lipids and carbohydrates.
C) They provide a rigid structure that prevents the uptake of excess water into cells.
D) They are constructed of polymers that are synthesized in the cytoplasm and then transported out of the cell for assembly.

E) All of the above
F) C) and D)

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A biologist ground up some plant leaf cells and then centrifuged the mixture to fractionate the organelles. Organelles in one of the heavier fractions could produce ATP in the light, whereas organelles in the lighter fraction could produce ATP in the dark. The heavier and lighter fractions are most likely to contain, respectively,


A) mitochondria and chloroplasts.
B) chloroplasts and peroxisomes.
C) peroxisomes and chloroplasts.
D) chloroplasts and mitochondria.
E) mitochondria and peroxisomes.

F) D) and E)
G) B) and E)

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Which of the following will be found in nearly all eukaryotic cells?


A) cell wall
B) mitochondria
C) chloroplast
D) nucleoid
E) capsule

F) B) and C)
G) A) and C)

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When disrupted cells are spun in a centrifuge at increasingly higher speeds, which of the following correctly lists the order in which cellular components will pellet?


A) ribosomes, nuclei, mitochondria
B) chloroplasts, ribosomes, pieces of membrane
C) nucleus, ribosomes, chloroplasts
D) nucleus, mitochondria, ribosomes

E) C) and D)
F) A) and B)

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Thylakoids, DNA, and ribosomes are all components found in


A) vacuoles.
B) chloroplasts.
C) mitochondria.
D) lysosomes.
E) nucleoli.

F) B) and C)
G) A) and B)

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Which of the following may be found in eukaryotic cells, but not in bacteria?


A) DNA
B) flagella
C) plasma membrane
D) ribosomes
E) endoplasmic reticulum

F) B) and C)
G) A) and E)

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What technique would be most appropriate to use to observe the movements of condensed chromosomes during cell division?


A) standard light microscopy
B) super-resolution microscopy
C) transmission electron microscopy
D) scanning electron microscopy

E) None of the above
F) All of the above

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One advantage of light microscopy over transmission electron microscopy is that


A) specimen preparation for light microcopy does not produce artifacts.
B) light microscopy provides for higher resolving power than transmission electron microscopy.
C) light microscopy provides for higher magnification than transmission electron microscopy.
D) light microscopy allows one to view dynamic processes in living cells.

E) B) and D)
F) A) and C)

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Which organelle often takes up much of the volume of a plant cell?


A) lysosome
B) vacuole
C) mitochondrion
D) Golgi apparatus
E) peroxisome

F) A) and C)
G) B) and D)

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Which cell would be best for studying lysosomes?


A) muscle cell
B) nerve cell
C) phagocytic white blood cell
D) bacterial cell

E) A) and C)
F) A) and D)

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Some key innovations in the evolution of eukaryotes from a prokaryotic ancestor are membrane-bound organelles. Which of the following organelles or features is likely to be a common component shared by both eukaryotic cells and their prokaryotic ancestors?


A) ribosomes
B) chloroplasts
C) mitochondria
D) nuclear envelope

E) B) and C)
F) A) and B)

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