Question 1

A mutation that changes a cysteine codon to a tryptophan codon is called a

Accepted Answer

A)  missense mutation.
B)  nonsense mutation.
C)  silent mutation.
D)  none of the above.E) None of the above
F) All of the above

Question 2

A step in the generation of knockout mutations in mice includes selection of embryonic stem (ES) cells that are

Accepted Answer

A)  resistant to G418 and resistant to ganciclovir.
B)  sensitive to G418 and resistant to ganciclovir.
C)  resistant to G418 and sensitive to ganciclovir.
D)  sensitive to G418 and sensitive to ganciclovir.E) B) and D)
F) A) and B)

Question 3

Linkage studies can map disease genes with a resolution of about one centimorgan.Typically,the physical distance of a DNA region this size is approximately:

Accepted Answer

A)  7.5 × 10² base pairs
B)  7.5 × 10³ base pairs
C)  7.5 × 10⁴ base pairs
D)  7.5 × 10⁵ base pairsE) B) and C)
F) None of the above

Question 4

Organisms that are considered polyploid have:

Accepted Answer

A)  more than one genome.
B)  more than two copies of each chromosome.
C)  only one copy of each chromosome.
D)  multiple copies of a specific chromosome.E) B) and C)
F) All of the above

Question 5

Sequencing methods were used to test the integrity of the cDNA library and when this was done you found many of the sequences were those found in introns.After troubleshooting you determined that the reason these intronic sequences were present was because during the construction of this cDNA library:

Accepted Answer

A)  genomic DNA was present.
B)  retroviral DNA was a contaminant.
C)  endonucleases should have been added.
D)  RNA polymerase was present.E) A) and B)
F) None of the above

Question 6

Describe some typical features of a restriction enzyme recognition sequence.

Accepted Answer

Restriction enzyme recognition sequences...

Question 7

Which of the following series of steps does the polymerase chain reaction follow in order to amplify DNA in a test tube?

Accepted Answer

A)  DNA denaturation,primer elongation,primer annealing
B)  primer annealing,DNA ligation,primer elongation
C)  primer elongation,primer annealing,DNA ligation
D)  DNA denaturation,primer annealing,primer elongationE) None of the above
F) B) and C)

Question 8

Microsatellites,which often vary in DNA sequence between individuals,are also called:

Accepted Answer

A)  single-nucleotide polymorphisms.
B)  nucleotide tandem replacements.
C)  short tandem repeats.
D)  autosomal dominant polymorphism.E) All of the above
F) A) and C)

Question 9

Which of the following is a functional element of a plasmid?

Accepted Answer

A)  origin of replication
B)  drug-resistance gene
C)  polylinker sequence
D)  all of the aboveE) A) and B)
F) A) and C)

Question 10

You are growing a population of cells that appear to be resistant to G418,a chemical that is normally toxic to cells.Upon further analysis of the cells' genome you find that it contains a genomic sequence that encodes the protein neomycin phosphotransferase.What would you do to test if this protein confers resistance to the G418 chemical?

Accepted Answer

A)  determine if the cells are also making β-galactosidase
B)  tag the protein with green fluorescent protein to see if it is degraded by G418
C)  label a fragment of the gene to see where it is expressed
D)  clone the gene into a G418-sensitive cell line,then treat these cells with G418E) C) and D)
F) A) and C)

Question 11

Modifying the DNA sequence of a gene of interest by appending to it a DNA sequence that encodes a stretch of amino acids recognized by a known monoclonal antibody is called:

Accepted Answer

A)  epitope tagging.
B)  in situ hybridization.
C)  polymerase chain reaction.
D)  next-generation sequencing.E) B) and D)
F) A) and C)

Question 12

How does genetic heterogeneity or polygenic traits make the identification of a disease gene more difficult?

Accepted Answer

Some inherited diseases result from a mu...

Question 13

A mutation in one gene that counteracts the effects of a mutation in another gene is known as a

Accepted Answer

A)  temperature-sensitive mutation.
B)  recessive mutation.
C)  conditional mutation.
D)  suppressor mutation.E) A) and B)
F) None of the above

Question 14

A haplotype is a set of closely linked genetic markers on a particular chromosome that tend to be inherited together.The genetic technique that looks at inheritance patterns and uses haplotypes in determining gene locations is:

Accepted Answer

A)  linkage mapping.
B)  linkage disequilibrium mapping.
C)  candidate gene approach.
D)  all of the aboveE) A) and B)
F) C) and D)

Question 15

Which human disease is the result of an inherited X-liked recessive mutation?

Accepted Answer

A)  Tay-Sachs disease
B)  Duchenne muscular dystrophy
C)  cystic fibrosis
D)  none of the aboveE) C) and D)
F) B) and D)

Question 16

In addition to sequencing,intronic sequences can be identified in a genomic DNA library using which of the following methods?

Accepted Answer

A)  Southern blotting
B)  in situ hybridization
C)  PCR
D)  Southern blotting and PCRE) A) and C)
F) A) and B)

Question 17

In the large-scale production of a particular human protein in E.coli cells,the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus.The purpose of this modification was to

Accepted Answer

A)  facilitate transfer of the cDNA into the E.coli cells.
B)  provide a promoter for the transcription of the cDNA in E.coli.
C)  facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel atoms.
D)  prevent degradation of the expressed protein by E.coli proteases.E) A) and B)
F) B) and C)

Question 18

In situ hybridization is a powerful tool used in gene expression studies because it provides the investigator with information pertaining to

Accepted Answer

A)  the cellular and tissue-specific localization of the mRNA encoded by a particular gene.
B)  the activity of the protein translated from a particular mRNA.
C)  the size of the mRNA transcript.
D)  all of the aboveE) A) and D)
F) A) and C)

Question 19

In RNA interference studies,the double-stranded RNA

Accepted Answer

A)  disrupts the target DNA sequence.
B)  results in the destruction of the target mRNA.
C)  destroys the target protein.
D)  all of the aboveE) A) and B)
F) A) and C)

Question 20

How can linkage analysis determine the position of genes on a chromosome?

Accepted Answer

Linkage analysis examines the frequency ...

Exam 2: Molecular Genetic Techniques

A haplotype is a set of closely linked genetic markers on a particular chromosome that tend to be inherited together.The genetic technique that looks at inheritance patterns and uses haplotypes in determining gene locations is:

Correct Answer
verified

Describe some typical features of a restriction enzyme recognition sequence.

Correct Answer
verified
Restriction enzyme recognition sequences...
View Answer

In addition to sequencing,intronic sequences can be identified in a genomic DNA library using which of the following methods?

Correct Answer
verified

How does genetic heterogeneity or polygenic traits make the identification of a disease gene more difficult?

Correct Answer
verified
Some inherited diseases result from a mu...
View Answer

Which of the following is a functional element of a plasmid?

Correct Answer
verified

Microsatellites,which often vary in DNA sequence between individuals,are also called:

Correct Answer
verified

A mutation that changes a cysteine codon to a tryptophan codon is called a

Correct Answer
verified

Correct Answer
verified

In situ hybridization is a powerful tool used in gene expression studies because it provides the investigator with information pertaining to

Correct Answer
verified

Which of the following series of steps does the polymerase chain reaction follow in order to amplify DNA in a test tube?

Correct Answer
verified

Linkage studies can map disease genes with a resolution of about one centimorgan.Typically,the physical distance of a DNA region this size is approximately:

Correct Answer
verified

In RNA interference studies,the double-stranded RNA

Correct Answer
verified

A step in the generation of knockout mutations in mice includes selection of embryonic stem (ES) cells that are

Correct Answer
verified

A mutation in one gene that counteracts the effects of a mutation in another gene is known as a

Correct Answer
verified

Correct Answer
verified

Modifying the DNA sequence of a gene of interest by appending to it a DNA sequence that encodes a stretch of amino acids recognized by a known monoclonal antibody is called:

Correct Answer
verified

In the large-scale production of a particular human protein in E.coli cells,the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus.The purpose of this modification was to

Correct Answer
verified

Which human disease is the result of an inherited X-liked recessive mutation?

Correct Answer
verified

How can linkage analysis determine the position of genes on a chromosome?

Correct Answer
verified
Linkage analysis examines the frequency ...
View Answer

Organisms that are considered polyploid have:

Correct Answer
verified

Exam 2: Molecular Genetic Techniques

A haplotype is a set of closely linked genetic markers on a particular chromosome that tend to be inherited together.The genetic technique that looks at inheritance patterns and uses haplotypes in determining gene locations is:

Correct AnswerverifiedShow Answer

Describe some typical features of a restriction enzyme recognition sequence.

Correct AnswerverifiedRestriction enzyme recognition sequences...View AnswerShow Answer

In addition to sequencing,intronic sequences can be identified in a genomic DNA library using which of the following methods?

Correct AnswerverifiedShow Answer

How does genetic heterogeneity or polygenic traits make the identification of a disease gene more difficult?

Correct AnswerverifiedSome inherited diseases result from a mu...View AnswerShow Answer

Which of the following is a functional element of a plasmid?

Correct AnswerverifiedShow Answer

Microsatellites,which often vary in DNA sequence between individuals,are also called:

Correct AnswerverifiedShow Answer

A mutation that changes a cysteine codon to a tryptophan codon is called a

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

In situ hybridization is a powerful tool used in gene expression studies because it provides the investigator with information pertaining to

Correct AnswerverifiedShow Answer

Which of the following series of steps does the polymerase chain reaction follow in order to amplify DNA in a test tube?

Correct AnswerverifiedShow Answer

Linkage studies can map disease genes with a resolution of about one centimorgan.Typically,the physical distance of a DNA region this size is approximately:

Correct AnswerverifiedShow Answer

In RNA interference studies,the double-stranded RNA

Correct AnswerverifiedShow Answer

A step in the generation of knockout mutations in mice includes selection of embryonic stem (ES) cells that are

Correct AnswerverifiedShow Answer

A mutation in one gene that counteracts the effects of a mutation in another gene is known as a

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Modifying the DNA sequence of a gene of interest by appending to it a DNA sequence that encodes a stretch of amino acids recognized by a known monoclonal antibody is called:

Correct AnswerverifiedShow Answer

In the large-scale production of a particular human protein in E.coli cells,the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus.The purpose of this modification was to

Correct AnswerverifiedShow Answer

Which human disease is the result of an inherited X-liked recessive mutation?

Correct AnswerverifiedShow Answer

How can linkage analysis determine the position of genes on a chromosome?

Correct AnswerverifiedLinkage analysis examines the frequency ...View AnswerShow Answer

Organisms that are considered polyploid have:

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
Restriction enzyme recognition sequences...
View Answer

Correct Answer
verified

Correct Answer
verified
Some inherited diseases result from a mu...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Linkage analysis examines the frequency ...
View Answer

Correct Answer
verified